abstract：:Yeast enolase is stabilized by its natural cofactor Mg(2+). This stabilization is ascribed to the reduced subunit dissociation of the holoprotein. Nevertheless, how Mg(2+) alters the unfolding mechanism has yet to be fully characterized. Here, we investigate the role of Mg(2+) in the denaturation mechanism and unfoldi...

journal_title：International journal of biological macromolecules

authors： Moreno-Vargas LM,Carrillo-Ibarra N,Arzeta-Pino L,Benítez-Cardoza CG

更新日期：2011-12-01 00:00:00

abstract：:In the present study, small-sized porous scaffolds were obtained from the freeze-drying of sodium hyaluronate/chitosan polyelectrolyte complexes. The obtained materials were characterized by a set of techniques including attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, swelling determin...

journal_title：International journal of biological macromolecules

authors： Coimbra P,Alves P,Valente TA,Santos R,Correia IJ,Ferreira P

更新日期：2011-11-01 00:00:00

abstract：:The synthesis of chitosan-graft-γ-cyclodextrin (Ch-g-γ-CD) using persulfate/ascorbic acid redox system was done and characterized by FTIR, XRD, TGA and SEM/EDX. The optimum yield of the copolymer was obtained using 16×10(-3) M γ-cyclodextrins (γ-CD), 2.8×10(-2) M ascorbic acid (AA), 1.8×10(-2) M K(2)S(2)O(8) and 0.1g ...

journal_title：International journal of biological macromolecules

authors： Mishra AK,Sharma AK

更新日期：2011-11-01 00:00:00

abstract：:Fused Toes Homolog (FTS) is a member of a group of proteins termed as E2 variants and this group of proteins lacks an active cysteine residue that is required for ubiquitin transfer. We have identified the expression of this protein in early neoplastic stages of cervical cancer and its translocation into nucleus from ...

journal_title：International journal of biological macromolecules

authors： Anandharaj A,Yu JR,Park WY

更新日期：2011-11-01 00:00:00

abstract：:The stability of enzymes with no reduction in their catalytic activity still remains a critical issue in industrial applications. Naturally occurring osmolytes are commonly used as protein stabilizers. In this study we have investigated the effects of sorbitol and trehalose on the structural stability and activity of ...

journal_title：International journal of biological macromolecules

authors： Azizi A,Ranjbar B,Khajeh K,Ghodselahi T,Hoornam S,Mobasheri H,Ganjalikhany MR

更新日期：2011-11-01 00:00:00

abstract：:Prephenate dehydrogenase (PDH) is a bacterial enzyme that catalyzes conversion of prephenate to 4-hydroxyphenylpyruvate through the oxidative decarboxylation pathway for tyrosine biosynthesis. This enzymatic pathway exists in prokaryotes but is absent in mammals, indicating that it is a potential target for the develo...

journal_title：International journal of biological macromolecules

authors： Ku HK,Do NH,Song JS,Choi S,Yeon SH,Shin MH,Kim KJ,Park SR,Park IY,Kim SK,Lee SJ

更新日期：2011-11-01 00:00:00

abstract：:Opuntia is a traditional plant from China with medicinal applications. In this experiment, polysaccharides from Opuntia Milpa Alta (MAPs) were analyzed using gas chromatograph-mass spectrometer (GC-MS) method and result showed that MAPs contained mannose (6.37%), rhamnose (14.94%), xylose (1.99%), arabinose (24.07%), ...

journal_title：International journal of biological macromolecules

authors： Chen Y,Zhao B,Huang X,Zhan J,Zhao Y,Zhou M,Guo L

更新日期：2011-11-01 00:00:00

abstract：:We have previously reported that amino-terminal extension sequence containing hexa-His facilitated refolding and assembly of hexameric nucleoside diphosphate kinase from extremely halophilic archaeon Halobacterium salinarum (NDK). In this study, we made various mutations in both the tag sequence and within NDK molecul...

journal_title：International journal of biological macromolecules

authors： Ishibashi M,Ida K,Tatsuda S,Arakawa T,Tokunaga M

更新日期：2011-11-01 00:00:00

abstract：:Two COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest ...

journal_title：International journal of biological macromolecules

authors： Fernandes JC,Borges M,Nascimento H,Bronze-da-Rocha E,Ramos OS,Pintado ME,Malcata FX,Santos-Silva A

更新日期：2011-10-01 00:00:00

abstract：:The purpose of this study is to clarify that the amino acid residues (Asp62 and Arg193) are responsible for the activity and stability of arginine kinase (AK). The amino acid residues Asp62 (D62) and Arg193 (R193) are strictly conserved in monomeric AKs and form an ion pair in the transition state analogue complex. In...

journal_title：International journal of biological macromolecules

authors： Liu N,Wang JS,Wang WD,Pan JC

更新日期：2011-10-01 00:00:00

abstract：:Arginine kinase (AK), a crucial enzyme in energy metabolism, buffers cellular ATP levels by catalyzing the reversible phosphoryl transfer between ATP and arginine. To better understand the role of Cys271 in conformational changes of AK from greasyback shrimp (Metapenaeus ensis), we replaced the residue with serine and...

journal_title：International journal of biological macromolecules

authors： Liu N,Wang JS,Wang WD,Pan JC

更新日期：2011-07-01 00:00:00

abstract：:α-chitin hydrogel/nano hydroxyapatite (nHAp) composite scaffold have been synthesized by freeze-drying approach with nHAp and α-chitin hydrogel. The prepared nHAp and nanocomposite scaffolds were characterized using DLS, SEM, FT-IR, XRD and TGA studies. The porosity, swelling, degradation, protein adsorption and biomi...

journal_title：International journal of biological macromolecules

authors： Kumar PT,Srinivasan S,Lakshmanan VK,Tamura H,Nair SV,Jayakumar R

更新日期：2011-07-01 00:00:00

abstract：:Antitussive drugs are amongst the most widely used medications worldwide; however no new class of drugs has been introduced into the market for many years. Herein, we have analyzed the water-extracted polymeric fraction (WE) of Glycyrrhiza glabra. This arabinogalactan protein enriched fraction, ≥ 85% of which gets pre...

journal_title：International journal of biological macromolecules

authors： Saha S,Nosál'ová G,Ghosh D,Flešková D,Capek P,Ray B

更新日期：2011-05-01 00:00:00

abstract：:Proteoglycan (P1) purified from Phellinus linteus has been reported to have anti-disease activities. The objectives of our research were to determine the anti-tumor effect and possible mechanisms of P1 on human cancer cells. Cell inhibition assay showed that P1 has an antiproliferative effect on HepG2, HT-29, NCI-H 46...

journal_title：International journal of biological macromolecules

authors： Li YG,Ji DF,Zhong S,Zhu JX,Chen S,Hu GY

更新日期：2011-04-01 00:00:00

abstract：:Sterilization of milk protein without heating is of great interest. Gamma irradiation is a very powerful method to decontaminated casein. Gamma-irradiation of proteins in aqueous media at doses higher than 5kGy is known to induce their aggregation (without oxygen) or degradation (in presence of oxygen). Camellia sinen...

journal_title：International journal of biological macromolecules

authors： Saloua KS,Salah K,Nasreddine B,Samia A,Mouldi S,Ahmed L

更新日期：2011-04-01 00:00:00

abstract：:The giant extracellular hemoglobin of Glossoscolex paulistus (HbGp) has a molecular mass (M) of 3600±100 kDa and a standard sedimentation coefficient (s20,w0) of 58 S, estimated by analytical ultracentrifugation (AUC). In the present work, further AUC studies were developed for HbGp, at pH 10.0, which favors oligomeri...

journal_title：International journal of biological macromolecules

authors： Carvalho FA,Santiago PS,Borges JC,Tabak M

更新日期：2011-01-01 00:00:00

abstract：:Chlorogenic acid (CA) is a well-known ester of caffeic acid present in some food. It is also an active component in traditional Chinese medicines which are used to treat various diseases, but the molecular basis of CA is not clear. In the present work, the proton selective relaxation rate and the affinity index were u...

journal_title：International journal of biological macromolecules

authors： Wang Y,Zhang T,Xu J,Du W

更新日期：2011-01-01 00:00:00

abstract：:Protein phosphorylations have essential regulatory roles in visual signaling. Previously, we found that phosphorylation of several proteins in the retina and retinal pigment epithelium (RPE) is involved in anti-apoptotic signaling under oxidative stress conditions, including light exposure. In this study, we used a ph...

journal_title：International journal of biological macromolecules

authors： Lee H,Chung H,Lee SH,Jahng WJ

更新日期：2011-01-01 00:00:00

abstract：:In the present study, for the first time we have investigated the interaction of anticancer drug mitoxantrone with histone H1 and core histone proteins in solution using fluorescence, UV/Vis, CD spectroscopy and thermal denaturation techniques. The results showed that mitoxantrone reduced the absorbencies of H1 and co...

journal_title：International journal of biological macromolecules

authors： Hajihassan Z,Rabbani-Chadegani A

更新日期：2011-01-01 00:00:00

abstract：:This research is aimed to develop cationic nanofibrous mats with improved cellular adhesion profiles and stability of three-dimensional fibrous structure as potential scaffolds for skin tissue engineering. Firstly, amino-remained chitosan-graft-poly (ɛ-caprolactone) (CS-g-PCL) was synthesized with a facile one-step ma...

journal_title：International journal of biological macromolecules

authors： Chen H,Huang J,Yu J,Liu S,Gu P

更新日期：2011-01-01 00:00:00

abstract：:Immunoscreening of a cDNA expression library of the Rhipicephalus (Boophilus) annulatus tick with purified rabbit anti-R annulatus salivary glands antigens polyclonal antibodies led to the identification of a 661bp sequence. The sequence includes an open reading frame of 543bp encoding a protein of 180 amino acids wit...

journal_title：International journal of biological macromolecules

authors： Shahein YE,El-Rahim MT,Hussein NA,Hamed RR,El-Hakim AE,Barakat MM

更新日期：2010-12-01 00:00:00

abstract：:Identification of biomarker proteins in the retina and retinal pigment epithelium (RPE) under oxidative stress may imply new insights into signaling mechanisms of retinal degeneration at the molecular level. Proteomic data from an in vivo mice model in constant light and an in vitro oxidative stress model are compared...

journal_title：International journal of biological macromolecules

authors： Lee H,Arnouk H,Sripathi S,Chen P,Zhang R,Bartoli M,Hunt RC,Hrushesky WJ,Chung H,Lee SH,Jahng WJ

更新日期：2010-12-01 00:00:00

abstract：:A novel diethoxy phosphoryl chitosan was successfully synthesized via Atherton-Todd reaction of chitosan with diethyl phosphite, and the characterization was carried out by FT IR, NMR, ICP, XRD, TG and SEM, respectively. The FT IR, (13)C NMR and (31)P NMR results showed that diethoxy phosphoryl group was grafted onto ...

journal_title：International journal of biological macromolecules

authors： Ma L,Li G,Li L,Liu P

更新日期：2010-11-01 00:00:00

abstract：:The sugar compositions, α-glucosidase inhibitory and α-amylase inhibitory activities of polysaccharides from leaves and flowers of green tea (Camellia sinensis) obtained by hot water extraction (HWE), boiled water extraction (BWE) and enzymatic extraction (EE) were investigated. The yields, sugar contents and monosacc...

journal_title：International journal of biological macromolecules

authors： Wang Y,Yang Z,Wei X

更新日期：2010-11-01 00:00:00

abstract：:Rhus laccase (RL) was covalently immobilised onto chitosan, and the effects of immobilisation on pH optimum, enzyme activity, thermostability, and re-use evaluated, using either N,N-dimethyl-p-phenylenediamine or 2,6-dimethoxyphenol as substrate. Immobilisation greatly enhanced enzyme thermostability, resulted in negl...

journal_title：International journal of biological macromolecules

authors： Wan YY,Lu R,Xiao L,Du YM,Miyakoshi T,Chen CL,Knill CJ,Kennedy JF

更新日期：2010-11-01 00:00:00

abstract：:Microwave synthesis of starch-graft-poly(ethylacrylate) was optimized to obtain efficient Zn(II) adsorbent that has been characterized using IR, XRD and SEM analyses. Using the sample, adsorption of Zn(II) was studied as a function of pH, sorbent dose, initial Zn(II) concentration, % grafting, temperature and ionic st...

journal_title：International journal of biological macromolecules

authors： Singh V,Maurya S

更新日期：2010-10-01 00:00:00

abstract：:In this study, spin-labelled ovalbumin (SL-OVA), free or entrapped in liposomes, was administered to mice subcutaneously (s.c.) or intravenously (i.v.) with the aim to determine the conditions for pharmacokinetic studies of spin-labelled proteins by EPR and to measure the time course of SL-OVA distribution in vivo in ...

journal_title：International journal of biological macromolecules

authors： Abramović Z,Brgles M,Habjanec L,Tomasić J,Sentjurc M,Frkanec R

更新日期：2010-10-01 00:00:00

abstract：:Ultrafine fibers were successfully fabricated from Channel catfish skin-extracted gelatin via electrospinning (ES). Important ES parameters, such as concentration of aqueous acid and fish gelatin solutions, and electric field intensity were examined to investigate the effects on the morphology of the gelatin nanofiber...

journal_title：International journal of biological macromolecules

authors： An K,Liu H,Guo S,Kumar DN,Wang Q

更新日期：2010-10-01 00:00:00

abstract：:Toward the development of biocomposites for packaging applications, the possibility of using kenaf cellulose (KC) was investigated in the production of low-density polyethylene (LDPE)/KC/polyethylene glycol (PEG) biocomposites. First, cellulose was extracted from the cell walls of kenaf-bast fibers. Then, different we...

journal_title：International journal of biological macromolecules

authors： Tajeddin B,Rahman RA,Abdulah LC

更新日期：2010-08-01 00:00:00

abstract：:Chlorocatechol 1,2-dioxygenase (1,2-CCD) is a non-heme iron protein involved in the intradiol cleavage of aromatic compounds that are recalcitrant to biodegradation. In particular, 1,2-CCD catalyzes the conversion of catechol and its halogenated derivatives to cis-cis muconic acid. In this study we describe a series o...

journal_title：International journal of biological macromolecules

authors： Melo FA,Araújo AP,Costa-Filho AJ

更新日期：2010-08-01 00:00:00

abstract：:In this study, the fluid uptake (swelling) kinetics and disintegrant properties of gellan gum in metronidazole tablets were evaluated in both simulated gastric and intestinal fluids (SGF and SIF respectively) without enzymes. The mechanical properties as well as the disintegration and dissolution profile of the tablet...

journal_title：International journal of biological macromolecules

authors： Emeje MO,Franklin-Ude PI,Ofoefule SI

更新日期：2010-08-01 00:00:00

abstract：:In this study, potato and bean starches were treated by gamma-irradiation up to 50kGy. Molecular structure and physicochemical properties of irradiated potato and bean starches were investigated. Microscopic observation under scanning electron microscope (SEM) and polarized microscope showed that some of potato and be...

journal_title：International journal of biological macromolecules

authors： Chung HJ,Liu Q

更新日期：2010-08-01 00:00:00

abstract：:Boron is reported in this study to stabilize the structure of heme proteins exposed to peroxides. The oxidized heme protein (15 microM) was treated with H(2)O(2) (10mM) in 1M glycine-NaOH buffer (pH 9.2) at 25 degrees C in absence/presence of boron, and characterized by visible absorption spectroscopy, gel exclusion c...

journal_title：International journal of biological macromolecules

authors： Ali S,Farooqi H,Prasad R,Naime M,Routray I,Yadav S,Ahmad F

更新日期：2010-08-01 00:00:00

abstract：:Four prescriptions, epimedium flavone plus propolis flavone (EF-PF), epimedium flavone plus propolis extracts (EF-PE), epimedium polysaccharide plus propolis flavone (EP-PF) and epimedium polysaccharide plus propolis extracts (EP-PE), were prepared and their immune-enhancing effects were compared. In test in vitro, th...

journal_title：International journal of biological macromolecules

authors： Fan Y,Hu Y,Wang D,Guo Z,Zhao X,Guo L,Zhao B,Zhang J,Wang Y,Nguyen TL

更新日期：2010-08-01 00:00:00

abstract：:Protein stability assessment of seven model proteins in the presence of low molecular weight polyethyleneimine (PEI, MW 2000 Da) was performed. Thermodynamic stability, monitored by circular dichroism (CD) spectroscopy, showed that the polymer did not have a major effect on the melting temperature (T(m)) of the basic ...

journal_title：International journal of biological macromolecules

authors： Mazzaferro L,Breccia JD,Andersson MM,Hitzmann B,Hatti-Kaul R

更新日期：2010-07-01 00:00:00

abstract：:Creatine kinase (CK) is a key enzyme involved in intracellular energy homeostasis. The distinct tissue distribution of muscle CK (MMCK) and brain CK (BBCK) implies that they function under conditions facing dissimilar environmental stresses. We found that MMCK and BBCK were significantly different in their stability a...

journal_title：International journal of biological macromolecules

authors： Gao YS,Zhao TJ,Chen Z,Li C,Wang Y,Yan YB,Zhou HM

更新日期：2010-07-01 00:00:00

abstract：:Fluorescence quenching data on interaction of a fungicide methyl thiophanate (MT) with human serum albumin (HSA) elucidated a primary binding site at sub-domain IIA. Stern-Volmer algorithm and double log plot revealed the binding affinity (K(a)) and capacity (n) of HSA as 1.65 x 10(4)M(-1) and 1.0 (r(2)=0.99), respect...

journal_title：International journal of biological macromolecules

authors： Saquib Q,Al-Khedhairy AA,Alarifi SA,Dwivedi S,Mustafa J,Musarrat J

更新日期：2010-07-01 00:00:00

abstract：:This work studied the effect of preparation conditions for electrospun fiber mats of Thai silk fibroin/type B gelatin (SF/GB) for controlled release applications. The increasing in applied voltage resulted in decreased average fiber size and narrow fiber size distribution. An increasing in silk fibroin content in blen...

journal_title：International journal of biological macromolecules

authors： Okhawilai M,Rangkupan R,Kanokpanont S,Damrongsakkul S

更新日期：2010-06-01 00:00:00

abstract：:In order to enhance the drug entrapment efficiency and improve the swelling behaviors of drug delivery system, Ca2+ crosslinking and freeze-thawing (FT) cycle techniques were used to prepare sodium alginate/poly(vinyl alcohol) (SA/PVA) hydrogel beads. The mixture solution of SA and PVA was firstly crosslinked with Ca2...

journal_title：International journal of biological macromolecules

authors： Hua S,Ma H,Li X,Yang H,Wang A

更新日期：2010-06-01 00:00:00

abstract：:It is well established that a sequence template along with the database is a powerful tool for identifying the biological function of proteins. Here, we describe a method for predicting the catalytic nature of certain proteins among the several protein structures deposited in the Protein Data Bank (PDB). For the prese...

journal_title：International journal of biological macromolecules

authors： Gupta V,Prakash NA,Lakshmi V,Boopathy R,Jeyakanthan J,Velmurugan D,Sekar K

更新日期：2010-04-01 00:00:00